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Short experimental timeline compared to ChIP-Seq
dA-tailing
Phage T7 RNA polymerase is a DNA-dependent RNA polymerase that has strict specificity for its corresponding double-stranded promoter
- Molecular cloning
Converted DNA can be purified via spin columns and used for PCR
Lysozyme 50 mg/mL Spec.:N9021/1 mL*5 Short experimental timeline compared toLysozyme is an enzyme used to break down bacterial cell walls to improve protein or nucleic acid extraction efficiency. Lysozyme is a glycoside hydrolase that catalyzes the hydrolysis of 1,4 beta linkages between N acetylmuramic acid and N acetyl D glucosamine residues in peptidoglycan, which is the major component of gram positive bacterial cell wall. This hydrolysis in turn compromises the integrity of bacterial cell walls causing lysis of the
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