SIRV-Set 1 (Iso Mix E0, E1, E2) and E2 represents the natural

and E2 represents the natural situation

Spike-in control for RNA extraction and RT

The TATAA ACTN3 assay will tell if you have 2

while Alu assay is preferred when sensitivity is priority

and normalization

SIRV-Set 1 (Iso Mix E0, E1, E2) and E2 represents the naturalSIRV Set 1: Isoform Mixes E0, E1 and E2 The isoform module is available in SIRV Set 1 (Cat. No. 025) in 3 SIRV mixes, termed E0, E1 and E2, with each mix containing all 69 SIRV isoform transcripts (from 7 SIRV genes) but in different concentration ratios. E0 is ideal for assessing the detection capabilities of a given RNA Seq workflow, since all 69 transcripts are present in equimolar concentrations, and their detection should be unbiased and not a