Leptosphaeria maculans Probe Realtime PCR Kit (without internal control) Nucleic Acids & Derivatives Parameter Details Nominal concentration (format)

Parameter Details Nominal concentration (format) 10 CFU per tube (NNE002) and 100 CFU per tube (NNE001) Tube count per kit 3 tubes of mycobacterium standard + 3 tubes DNA Dilution Buffer per kit DNA Dilution Buffer (DDB) 1

Ct of NCS should be larger than the mean Ct of the lowest standard (or below LOQ if the LOQ is proven lower than the lowest standard)

Q5: Is there a calibration feature

and data analysis settings for ABI7500 (can be adapted to other instruments)

as this may reduce PCR efficiency

Leptosphaeria maculans Probe Realtime PCR Kit (without internal control) Nucleic Acids & Derivatives Parameter Details Nominal concentration (format)Description Leptosphaeria maculans Probe Realtime PCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe based real time PCR. Purified DNA is directly amplified using sequence specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real time monitoring of amplification. The resulting signals may be used for comparative