Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Iron Size:500g 9 Chloramphenicol 0

9 Chloramphenicol 0

Nitrogen is provided by ammonium chloride final osmolarity of the medium is maintained by NaCl

Synthetic Defined Minimal Media

2 at 25°C Suspend 20 gram powdered medium in 1 litre distilled water

49 Suspend 7

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Iron Size:500g 9 Chloramphenicol 0Ferric chloride, FeCl3, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Iron or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room